FIG. 5.

ΔprsA2 mutants are fully viable in broth culture despite an increased abundance of bacterial cytosol-associated proteins in culture supernatants. (A) Broth culture growth curves of the wild type and the ΔprsA2 mutant as measured by plating dilutions of bacterial culture onto solid medium at the indicated time points. Data represent results from one of three experiments performed in duplicate. •, wild type; ○, ΔprsA2 mutant. (B) Bacterial Live/Dead staining as performed on log-phase cultures of the wild type or a ΔprsA2 mutant. Live bacterial cells are stained with Cyto9 (green), while dead or membrane-compromised bacterial cells are stained with propidium iodide (red). The percentage of cells positive for propidium iodide (red) was determined by counting the number of total cells in a minimum of 10 randomly chosen fields from two independent experiments. Statistical significance was determined using a Student t test (P > 0.05).