FIG. 6.

Two-dimensional electrophoresis of bacterial exoproteins in the presence of PrfA activation reveals significantly altered protein profiles for ΔprsA2 mutants. Supernatant fractions derived from prfA(L140F) or ΔprsA2 prfA(L140F) mutant strains were TCA precipitated and subjected to two-dimensional SDS-PAGE, thereby revealing a significantly altered protein profile for the ΔprsA2 prfA(L140F) strain (B) in comparison to the prfA(L140F) strain with the wild-type prsA2 strain (A). As previously noted, the prfA(L140F) strain supernatant protein profile (A) is distinct from that of wild-type supernatants in which PrfA is not mutationally activated (Fig. 3A).