TABLE 1.
Bacterial strains and plasmids used in this study
| Strain | Description | Designation | Reference |
|---|---|---|---|
| TOP10 | E. coli host strain used for recombinant pPL2 plasmids | ||
| SM10 | E. coli host strain for conjugation of pPL2 plasmids | ||
| NF-L100 | L. monocytogenes 10403S parent strain | ||
| NF-L1167 | 10403S actA-gus-neo prfA(L140F) | L140F strain | 116 |
| NF-L1651 | 10403S with ΔprsA2::erm | ΔprsA2 mutant | 1 |
| NF-L1656 | NF-L1651 (ΔprsA2::erm) with integrated pPL2-prsA2 (pNF1255) | ΔprsA2 + pPL2-prsA2 mutant | 1 |
| NF-L1483 | HEL 402-10403S with a prsA1 in-frame deletion | ΔprsA1 mutant | 1 |
| NF-L1637 | NF-L1167 [prfA(L140F)] with ΔprsA2::erm | prfA(L140F) ΔprsA2 mutant | 1 |
| NF-L1631 | NF-L1438 (ΔprsA1) transduced with ΔprsA2::erm | ΔprsA1 ΔprsA2 mutant | This work |
| NF-L1605 | 10403S with a ΔhtrA in-frame deletion | ΔhtrA mutant | 113 |
| NF-L1633 | NF-L1605 (ΔhtrA) transduced with ΔprsA2::erm | ΔhtrA ΔprsA2 mutant | This work |
| NF-L1665 | NF-L1633 (ΔhtrA ΔprsA2) with integrated pPL2-prsA2 (pNF1255) | ΔhtrA ΔprsA2 + pPL2-prsA2 mutant | This work |
| NF-L1670 | NF-L1651 (ΔprsA2::erm) with integrated pPL2-PprsA2-prsA1 (pNF1611) | ΔprsA2 + pPL2-PprsA2-prsA1 mutant | This work |
| pNF1255 | pPL2 containing the prsA2 open reading frame for complementation | pPL2-prsA2 mutant | 1 |
| pNF1611 | pPL2 containing PprsA2-prsA1 SOE product | pPL2-PprsA2-prsA1 mutant | This work |