TABLE 5.
Proteins present in ΔprsA2 prfA(L140F) mutant gels but absent from prfA(L140F) gels
| Function or functional class | Gene no. | Protein name | Protein description | No. of peptide matches | % coverage | Protein score | Reference(s) |
|---|---|---|---|---|---|---|---|
| Virulence factors | lmo1786 | InlC | Internalin C | 8 | 29 | 568 | 19 and 24 |
| lmo0204 | ActA | Actin assembly-inducing protein | 7 | 33 | 1819 | 18 and 54 | |
| lmo0202 | LLO | Listeriolysin-O | 12 | 44 | 1444 | 83 and 86 | |
| lmo0205 | PlcB | Phospholipase-C | 3 | 17 | 36 | 92 and 101 | |
| lmo0203 | Mpl | Zinc metalloproteinase precursor | 2 | 9 | 179 | 63, 64, 75, and 76 | |
| Cell surface and cell wall metabolism | lmo2505 | P45 | Peptidoglycan lytic protein | 9 | 23 | 4374 | 85 |
| lmo2522 | LysM domain-containing protein | 5 | 34 | 736 | 74 | ||
| lmo1547 | MreC | Rod shape-determining protein | 4 | 11 | 468 | 98 | |
| Transport/binding proteins and lipoproteins | lmo2416 | ABC transporter, substrate binding protein | 2 | 11 | 204 | ||
| lmo0369 | Conserved hypothetical protein | 2 | 14 | 109 | |||
| lmo1388 | TcsA | CD4+ T cell-stimulating antigen (lipoprotein) | 2 | 6.9 | 227 | 74 | |
| lmo1757 | Putative lipoprotein | 2 | 8.4 | 395 | |||
| lmo2637 | Hypothetical pheromone-like protein | 3 | 13 | 687 | 74 | ||
| lmo1847 | ABC transporter, manganese binding protein | 4 | 15 | 642 | |||
| lmo1002 | PtsH | PTS phosphocarrier protein (Hpr) | 4 | 41 | 782 | 14 | |
| Protein folding | lmo1474 | GrpE | Heat shock protein/cochaperone | 4 | 36 | 246 | |
| lmo1267 | Tig | Trigger factor (prolyl isomerase) | 8 | 27 | 1379 | 7 | |
| Metabolism | |||||||
| Carbohydrates | lmo2455 | Eno | Enolase | 16 | 50 | 3741 | 81 |
| lmo2367 | Pgi | Glucose-6-phosphate isomerase | 2 | 6.2 | 225 | ||
| lmo2456 | Pgm | Phosphoglycerate mutase, 2,3-biphosphoglycerate independent | 10 | 31 | 1668 | ||
| lmo2459 | Gap | Glyceraldehyde 3-phosphate dehydrogenase | 2 | 8.6 | 416 | 81 | |
| Lipids | lmo1808 | FabD | Malonyl coenzyme A acyl carrier protein transacylase | 3 | 13 | 296 | |
| Coenzymes | lmo2101 | Pyridoxine biosynthesis protein | 4 | 22 | 744 | ||
| Amino acids | lmo2414 | SufD | Hypothetical FeS assembly protein | 3 | 11 | 246 | |
| Nucleic acids | lmo2611 | Adk | Adenylate kinase | 5 | 26 | 875 | |
| Detoxification/adaptation to atypical conditions | lmo0927 | IspB | Sulfatase family protein | 6 | 13 | 820 | 110 |
| lmo2785 | Kat | Catalase | 2 | 5.1 | 102 | 26 and 58 | |
| lmo1439 | Sod | Superoxide dismutase | 6 | 37 | 2726 | 2 and 100 | |
| lmo2468 | ClpP | ATP-dependent Clp protease proteolytic subunit | 3 | 20 | 602 | 33 and 34 | |
| lmo0943 | Fri | Nonheme iron-binding ferritin | 4 | 36 | 181 | 25 | |
| Regulation and transcription | lmo0443 | Transcriptional regulator (putative) | 8 | 28 | 1212 | ||
| lmo1280 | CodY | Transcriptional repressor | 7 | 37 | 1050 | 4 | |
| lmo1496 | GreA | Transcription elongation factor | 5 | 44 | 606 | ||
| Protein synthesis | lmo0239 | CysS | Cysteinyl-tRNA synthetase | 2 | 7.6 | 284 | 61 |
| lmo1657 | Tsf | Translation elongation factor Ts | 7 | 26 | 853 | ||
| lmo2654 | Fus | Elongation factor G | 2 | 4.6 | 479 | ||
| lmo1314 | Frr | Ribosome recycling factor | 5 | 31 | 676 | ||
| lmo0250 | RplJ | 50S ribosomal protein L10 | 4 | 24 | 617 | 27 | |
| lmo0251 | RplL | 50S ribosomal protein L7/L12 | 2 | 20 | 305 | ||
| Amino acid synthesis | lmo0223 | CysK | Cysteine synthase A | 8 | 43 | 631 | 22 |
| Specific pathways | lmo2556 | FbaA | Fructose bisphosphate aldolase | 5 | 18 | 1045 | |
| lmo1339 | Glucokinase | 2 | 8.4 | 363 | |||
| lmo0429 | Glycosyl hydrolase | 2 | 8 | 205 | |||
| lmo0811 | Carbonic anhydrase | 2 | 11 | 399 |