Skip to main content
. 2010 Sep 7;78(11):4532–4541. doi: 10.1128/IAI.00802-10

FIG. 5.

FIG. 5.

ARAP2 promotes entry of Listeria in part by inactivating host Arf6 GTPase. (A) Depletion of Arf6 restores bacterial internalization to cells expressing ARAP2.R278K. HeLa cells were transfected with control or Arf6 (A6) siRNA and then with plasmids expressing Flag-tagged wild-type (WT), R728K, or K1190P alleles of ARAP2. Entry of Listeria was measured using a fluorescence microscopy-based assay (38) (see Materials and Methods). Data are averages ± SD from three experiments. *, P < 0.001 compared to the ARAP2wt plus control siRNA condition. (B) Expression of ARAP2 alleles. Expression of Flag-tagged ARAP2 proteins was measured using confocal microscopy (see Materials and Methods). P = 0.59 (not significant). (C) Depletion of Arf6. siRNA-mediated depletion of Arf6 was confirmed by Western blotting.