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. 2010 Aug 9;78(11):4477–4487. doi: 10.1128/IAI.00501-10

FIG. 1.

FIG. 1.

Region-specific Lmp1 antibodies detect the target recombinant protein and the native Lmp1. (A) Schematic drawing of region-specific Lmp1 proteins. Positions of leader peptide, N-terminal region (Lmp1N), a middle region (Lmp1M) with seven tandem peptide-repeats of 54 amino acids, and a C-terminal region (Lmp1C) containing several tetratricopeptide repeat (TPR) motifs are indicated. (B) Antibody response against region-specific truncated Lmp1 proteins. The recombinant proteins were probed with region-specific antisera, revealing detection of Lmp1N (34 kDa), Lmp1M (38 kDa), and Lmp1C (37 kDa) without detectable cross-reactivity. (C) Region-specific Lmp1 antibodies recognize the native protein. B. burgdorferi lysates were immunoblotted with antisera against region-specific truncated Lmp1 proteins. lmp1, lmp1 mutant.