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. 2010 Sep 7;78(11):4613–4624. doi: 10.1128/IAI.00533-10

FIG. 4.

FIG. 4.

(A) ELISPOT IFN-γ and IL-4 responses in HLA-A2, C57BL/6, and BALB/c mice immunized by three subcutaneous injections of MAP-1 vaccine in Montanide ISA 51. Freshly isolated spleen cells were pooled from five mice per group and cultured in the presence of MAP-1 or individual peptides (10 μg/ml). Data are presented as mean numbers of antigen-specific IFN-γ/IL-4 SFC per million spleen cells, and error bars reflect the standard deviation of triplicate samples. (B) ELISPOT IFN-γ and IL-4 responses in C57BL/6 and BALB/c mice immunized by three subcutaneous injections of MAP-2 vaccine in Montanide ISA 51. Freshly isolated spleen cells from five mice per group were pooled and cultured in the presence of MAP-2 or individual peptides (10 μg/ml). Data are presented as mean numbers of antigen-specific IFN-γ/IL-4 SFC per million spleen cells, and error bars reflect the standard deviations of triplicate samples.