FIG. 4.

Amino acid frequencies at each polymorphic site are shown for the entire sequenced region. Blue indicates a common allele, red indicates the second most common allele, and other colors indicate additional rarer alleles at some sites. Asterisks indicate the positions at which the polymorphisms were observed more commonly for children with clinical malaria than for those with asymptomatic infections, or vice versa, at a P value of <0.10 level. After correction for multiple testing by the Bonferroni method (6), this remained significant only for amino acid residues at position 230 (P < 0.001).