FIG. 4.

Disruptions in glycolysis inhibit Ipa synthesis. (A) Strains tested were 2457T (WT), AGS120 (csrA), AGS220 (pfkA), AGS190 (cra), AGS120/pQCsrA, and AGS220, carrying either the empty vector (pWSK29) or the cloned pfkA gene (pWPkfA). Total cell extracts were prepared from exponential-phase cells and then analyzed by SDS-PAGE and immunoblotting with mouse monoclonal anti-IpaB or mouse monoclonal anti-IpaC antibody. Lysates from equal numbers of cells as determined by optical density were used.