TABLE 1.
Strains and plasmids
| Strain or plasmid | Description | Source and/or reference |
|---|---|---|
| E. coli strains | ||
| JW0078 | cra::Kan | Keio collection (1) |
| JW1899 | uvrY::Kan | Keio collection |
| JW2757 | barA::Kan | Keio collection |
| JW3221 | csrD::Kan | Keio collection |
| JW3887 | pfkA::Kan | Keio collection |
| TRMG1655 | csrA::Kan | 44 |
| RG1-B | csrB::Cm | 15 |
| TWMG1655 | csrC::Tet | 68 |
| S. flexneri 2a strains | ||
| 2457T | Wild type | Walter Reed Army Institute of Research |
| AGS100 | 2457T barA::Kan | This study |
| AGS110 | 2457T uvrY::Kan | This study |
| AGS120 | 2457T csrA::Kan | This study |
| AGS130 | 2457T csrB::Cm | This study |
| AGS140 | 2457T csrC::Tet | This study |
| AGS150 | 2457T csrD::Kan | This study |
| AGS190 | 2457T cra::Kan | This study |
| AGS220 | 2457T pfkA::Kan | This study |
| Plasmids | ||
| pGEM-T Easy | PCR cloning vector | Promega |
| pQE2 | Cloning vector; IPTG-inducible expression from T5 promoter | Qiagen |
| pWKS30 | Low-copy-number cloning vector | 63 |
| pWSK29 | Low-copy-number cloning vector | 63 |
| pQCsrB | 2457T csrB in pQE2 | This study |
| pQCsrC | 2457T csrC in pQE2 | This study |
| pQCsrA | 2457T csrA in pQE2 | This study |
| pWCsrA | 2457T csrA in pWSK29 | This study |
| pWCra | 2457T cra in pWKS30 | This study |
| pWPfkA | 2457T pfkA in pWSK29 | This study |