TABLE 2.

Primers used in this study

Primer	Sequence
csrA-fwd-BseRI	5′-ATCTGAGGAGCAGCAAAGTATGCTGATTCTG-3′
csrA-rev-HindIII	5′-AAGCTTGGGTGCGTCTCACCGATAAAG-3′
pQCsrB-fwd	5′-GAATTCTATCTTCGTCGACAGG-3′
pQCsrB-rev	5′-AAGCTTAAAACTGCCGCGAAGGATAGC-3′
pQCsrC-fwd	5′-GAATTCCAAAGGCGTAAAGTAGCACCC-3′
pQCsrC-rev	5′-AAGCTTCTTTTGCATGACCTTTGCTGCG-3′
pWPfkA-fwd	5′-GTGGACAGGGAGGGTAAACGGTCTATG-3′
pWPfkA-rev	5′-GCGGCCGCCTTGCGGGTATATGTTGAGGG-3′
pWCra-fwd	5′-GAATTCTGCGAAATCAGTGGGAAC-3′
pWCra-rev	5′-GCGGCCGCGTTGGCAGCATTACCTTG-3′
For real-time PCR
virB2-for	5′-TCCAATCGCGTCAGAACTTAACT-3′
virB2-rev	5′-CCTTTAATATTGGTAGTGTAGAACTAAGAGATTC-3′
virB2 probe	FAM-5′-AGGACTTGAAAAGGC-3′-MGBNFQ
VirF-fwd	5′-TCGATAGCTTCTTCTCTTAGTTTTTCTG-3′
VirF-rev	5′-GAAAGACGCCATCTCTTCTCGAT-3′
VirF probe	FAM-5′-TCAGATAAGGAAGATTGTTGAAA-3′-MGBNFQ