FIG. 2.

Differential gene expression of unfed and HZ-fed human monocytes as measured by cDNA macroarray. Unfed (A) and HZ-fed (B) immunopurified human monocytes were incubated for 4 h following 2 h of phagocytosis. After isolation, the pooled RNAs of monocytes obtained from 3 different healthy blood donors were converted into ³³P-labeled cDNA probes. The probes were hybridized to a Panorama Human Cytokine Gene Array filter containing 375 different human cytokine-related genes spotted in duplicate. Hybridization was detected using a phosphorimager. The most strongly upregulated genes are labeled. The data were obtained from one representative of two independent experiments.