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. 2010 Aug 23;78(11):4912–4921. doi: 10.1128/IAI.00455-10

FIG. 5.

FIG. 5.

Real-time RT-PCR analysis of HZ-related chemokines and IL-1RA gene expression in 15-HETE-treated human monocytes. Immunopurified monocytes were treated for 6 h with different doses of 15-HETE (1 μM and 10 μM). The expression of ENA-78, GROα, GROβ, GROγ, IL-8, MCP-1, MIP-1α, and MIP-1β (A) and IL-1RA (B) genes was measured. Threshold cycle values were normalized to GAPDH, and the data are expressed as the ratio between relative gene expression levels of HETE-treated versus untreated monocytes. Measurements were done in triplicate, and the data are presented as means plus SEM. A one-way ANOVA and Tukey's test were used for statistical analysis: 1 μM 15-HETE-treated versus untreated cells, no significant increase (P > 0.05); 10 μM 15-HETE-treated versus untreated cells, significant increases for ENA-78 (P = 0.001), GROα (P = 0.012), GROβ (P = 0.040), and IL-8 (P = 0.001); all other genes were not significantly modulated (P > 0.05).