FIG. 6.

Intermediate-term HSP27 and HSP70 protein expression in unfed and HZ-fed human immunopurified monocytes. Monocytes were incubated for 9 h following incubation with and without HZ and phagocytosis. Alternatively, cells were treated for 9 h with 1 μM gliotoxin. Thereafter, HSP27 (A) and HSP70 (B) proteins were detected in cell lysates by Western blotting (top; representative images) and analyzed by optical densitometry of 27- and 70-kDa bands (bottom; mean values plus SEM of arbitrary densitometric units from three independent experiments). The data were analyzed by Student's t test. (A) HZ-fed versus unfed cells, P < 0.05; gliotoxin-treated versus unfed cells, P < 0.005; HZ-fed versus gliotoxin-treated cells, P < 0.002. (B) No significant differences.