FIG. 2.

Anteiso-BCFA enhance intracellular growth of L. monocytogenes in RAW 264.7 macrophages. Stationary-phase bacterial cultures were used to infect RAW 264.7 macrophages at an MOI of 5. At the times indicated, host cells were lysed to enumerate intracellular CFU. (A) No 2MB was used for the growth of bacterial inoculum or during infection. (B) Supplementation of 2MB was used for the growth of bacterial inoculum and during infection for the synthesis of anteiso-BCFA. Averages of triplicates from a single experiment are plotted, with error bars representing standard deviations. The data shown here are representative of at least 3 independent experiments. Statistically significant differences between the WT and BKD-1 are represented by asterisks (**, P < 0.01; ***, P < 0.001). P values were determined using the unpaired t test in GraphPad Prism 5 software.