FIG. 3.

Binding of serum proteins by B. lusitaniae isolates. B. lusitaniae isolates as well as control strains B. garinii G1, B. burgdorferi B31-e2, B. spielmanii A14S, and B. afzelii FEM1-D15 incubated in NHS-EDTA were extensively washed with PBSA containing 0.05% Tween 20, and bound proteins were eluted using 0.1 M glycine (pH 2.0). The last wash (w) and the eluate fraction (e) obtained from each isolate were separated using nonreducing 12.5% SDS-PAGE, transferred to nitrocellulose, and probed with a polyclonal anti-CFH antiserum (Calbiochem, Darmstadt, Germany). Purified CFH was used as a positive control. The mobility of the marker proteins (Precision Plus protein standard) is indicated on the left. The band corresponding to FHL1 is indicated by an asterisk.