FIG. 5.

Identification of CFH-binding proteins of B. lusitaniae isolates. Cell lysates (30 μg each) obtained from diverse B. lusitaniae isolates and control strains B. burgdorferi sensu stricto LW2 and B. garinii G1 were separated by 10% Tris-Tricine SDS-PAGE and transferred to nitrocellulose. The membranes were incubated with NHS as a source for CFH, and binding of the proteins was detected by a polyclonal anti-CFH serum. The identified CRASP proteins, CspA, CspZ, and ErpP of B. burgdorferi LW2 and OspE of B. lusitaniae MT-M8, are indicated on the right, and the mobility of the marker proteins is indicated on the left.