TABLE 2.
Oligonucleotides used in this study
| Primer | Sequence (5′ to 3′)a |
|---|---|
| UHB(+) | GTTGGTTAAAATTACATTTGCG |
| E470(−) | CTAGTGATATTGCATATTCAG |
| OspE 34(+) | GCTGTTTTTGCACTCCCGGGTTCTTGTGGAAAGTTT |
| ErpP Hind(−) | CAGCACAAACAATCAAAGCTTTTTTATTCATAATTATTC |
| BsCRASP-3 145(+) BamHI | GCTGTTTTTGCACTGTTTGGATCCTGTGGAAATTTTAC |
| BsCRASP-3 nc(−) | ATTCATAATTATTCTCTTCTCGAGTTTGAATTTCTA |
| OspE 55(+) BamHI | GTTTGTCCTGATAAGTGGATCCAAAACTGATGAAAGC |
| OspE 3nc(−) XhoI | CTTTTTTATTCATAATTATTCTCGAGTATACTTTAAACTTCTA |
| FlaB lusF | CACCAGCATCACTTTCAGGATCTCAAGC |
| FlaB lusR | GAGCTCCTTCTTGTTGAACACC |
| OspE F2 | GGGGGATCATTTAAAACTGGTATG |
| OspE R2 | CTTCTTAAACTCTTCTAATGGTATTGC |
a
Nucleotides underlined indicate introduced substitutions of the respective residues to generate an appropriate restriction site.