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. 2010 Aug 23;78(11):4500–4510. doi: 10.1128/IAI.00417-10

FIG. 6.

FIG. 6.

Replication of P. aeruginosa strain PAO1 or the triple-effector mutant of PAO1 (ΔexoS ΔexoT ΔexoY) complemented with exoS or domain mutant forms of exoS within human corneal epithelial cells. Data are shown as the percent increases in number of viable intracellular bacteria over a 4-h time window (i.e., from 4 to 8 h postinoculation with gentamicin in the extracellular medium). Corneal epithelial cells were exposed to 2 × 107 CFU of P. aeruginosa strain PAO1 or plasmid-complemented strains for 3 h followed by a 1- or 5-h gentamicin treatment (4- and 8-h time points, respectively) before cell lysis and enumeration of viable intracellular bacteria. *, significantly different from results for PAO1 wild type; #, significantly different from results for ΔexoSTY mutant complemented with exoS; a, significantly different from results for ΔexoSTY mutant complemented with pUCP18; b, not significantly different from results for ΔexoSTY mutant complemented with pUCP18; c, significantly different from results for ΔexoSTY mutant complemented with the ADPRT domain mutant form of exoS (E318D).