PAO1 wild type |
Complete type 3 secretion system (T3SS); expresses ExoS, ExoT, and ExoY |
50 |
PAO1 ΔexoS ΔexoT ΔexoY
|
Deficient in known T3SS effectors |
50 |
PAO1 ΔexoS
|
Deficient in ExoS |
50 |
PAO1 ΔexoT
|
Deficient in ExoT |
50 |
PAO1 ΔexoY
|
Deficient in ExoY |
50 |
PAO1 ΔexoS ΔexoT
|
Deficient in ExoS and ExoT |
50 |
PAO1 ΔexoS ΔexoY
|
Deficient in ExoS and ExoY |
50 |
PAO1 ΔexoT ΔexoY
|
Deficient in ExoT and ExoY |
50 |
PAO1 exsA::Ω |
Cannot activate T3SS |
54 |
pUCP18 |
Vector control for complementation |
2, 41, 42, 44
|
pUCP_exoS
|
Allows complementation with fully functional exoS
|
2, 41, 42, 44
|
pUCP_exoS(E381D) |
Allows complementation of exoS with mutant ADP-r activity domain |
2, 41, 42, 44
|
pUCP_exoS(R146K) |
Allows complementation of exoS with mutant GAP activity domain |
2, 41, 42, 44
|
pUCP_exoS(E379D/E381D/R146K) |
Allows complementation of exoS with mutant ADP-r and GAP domains |
2, 41, 42, 44
|
pUCP_exoS(Δ51-77) |
Allows complementation of exoS with a mutant membrane localization domain |
2, 41, 42, 44
|
PAO1 ΔexoS ΔexoT ΔexoY_pUCP18 |
Deficient in all known T3SS effectors. Vector control for complementation |
This study |
PAO1 ΔexoS ΔexoT ΔexoY_pUCP_exoS
|
Deficient in all known T3SS effectors; complemented with fully functional version of exoS
|
This study |
PAO1 ΔexoS ΔexoT ΔexoY_pUCP_exoS(E381D) |
Deficient in all known T3SS effectors; complemented with exoS with mutant ADP-r activity domain |
This study |
PAO1 ΔexoS ΔexoT ΔexoY_pUCP_exoS(R146K) |
Deficient in all known T3SS effectors; complemented with exoS with mutant Rho-GAP activity domain |
This study |
PAO1 ΔexoS ΔexoT ΔexoY_pUCP_exoS(E379D/E381D/R146K) |
Deficient in all known T3SS effectors; complemented with exoS with mutant ADP-r and Rho-GAP domains |
This study |
PAO1 ΔexoS ΔexoT ΔexoY_pUCP_exoS(Δ51-77) |
Deficient in all known T3SS effectors; complemented with exoS with mutant membrane localization domain |
This study |
PAO1 ΔexoS_pUCP18 |
Deficient only in ExoS; empty vector control for complementation |
This study |
PAO1 ΔexoS_pUCP_exoS
|
Deficient only in ExoS; complemented with a wild-type version of exoS
|
This study |
PAO1 ΔexoS_pUCP_exoS(E381D) |
Deficient only in ExoS; complemented with exoS with mutant ADP-r activity domain |
This study |
PAO1 ΔexoS_pUCP_exoS(R146K) |
Deficient only in ExoS; complemented with exoS with mutant Rho-GAP activity domain |
This study |
PAO1 ΔexoS_pUCP_exoS(E379D/E381D/R146K) |
Deficient only in ExoS; complemented with exoS with mutant ADP-r and Rho-GAP domains |
This study |
PAO1 ΔexoS_pUCP_exoS(Δ51-77) |
Deficient only in ExoS; complemented with exoS with mutant membrane localization domain |
This study |