TABLE 1.
Bacterial strains and plasmids used in this study
| Strain or plasmid | Relevant description | Reference or source |
|---|---|---|
| PAO1 wild type | Complete type 3 secretion system (T3SS); expresses ExoS, ExoT, and ExoY | 50 |
| PAO1 ΔexoS ΔexoT ΔexoY | Deficient in known T3SS effectors | 50 |
| PAO1 ΔexoS | Deficient in ExoS | 50 |
| PAO1 ΔexoT | Deficient in ExoT | 50 |
| PAO1 ΔexoY | Deficient in ExoY | 50 |
| PAO1 ΔexoS ΔexoT | Deficient in ExoS and ExoT | 50 |
| PAO1 ΔexoS ΔexoY | Deficient in ExoS and ExoY | 50 |
| PAO1 ΔexoT ΔexoY | Deficient in ExoT and ExoY | 50 |
| PAO1 exsA::Ω | Cannot activate T3SS | 54 |
| pUCP18 | Vector control for complementation | 2, 41, 42, 44 |
| pUCP_exoS | Allows complementation with fully functional exoS | 2, 41, 42, 44 |
| pUCP_exoS(E381D) | Allows complementation of exoS with mutant ADP-r activity domain | 2, 41, 42, 44 |
| pUCP_exoS(R146K) | Allows complementation of exoS with mutant GAP activity domain | 2, 41, 42, 44 |
| pUCP_exoS(E379D/E381D/R146K) | Allows complementation of exoS with mutant ADP-r and GAP domains | 2, 41, 42, 44 |
| pUCP_exoS(Δ51-77) | Allows complementation of exoS with a mutant membrane localization domain | 2, 41, 42, 44 |
| PAO1 ΔexoS ΔexoT ΔexoY_pUCP18 | Deficient in all known T3SS effectors. Vector control for complementation | This study |
| PAO1 ΔexoS ΔexoT ΔexoY_pUCP_exoS | Deficient in all known T3SS effectors; complemented with fully functional version of exoS | This study |
| PAO1 ΔexoS ΔexoT ΔexoY_pUCP_exoS(E381D) | Deficient in all known T3SS effectors; complemented with exoS with mutant ADP-r activity domain | This study |
| PAO1 ΔexoS ΔexoT ΔexoY_pUCP_exoS(R146K) | Deficient in all known T3SS effectors; complemented with exoS with mutant Rho-GAP activity domain | This study |
| PAO1 ΔexoS ΔexoT ΔexoY_pUCP_exoS(E379D/E381D/R146K) | Deficient in all known T3SS effectors; complemented with exoS with mutant ADP-r and Rho-GAP domains | This study |
| PAO1 ΔexoS ΔexoT ΔexoY_pUCP_exoS(Δ51-77) | Deficient in all known T3SS effectors; complemented with exoS with mutant membrane localization domain | This study |
| PAO1 ΔexoS_pUCP18 | Deficient only in ExoS; empty vector control for complementation | This study |
| PAO1 ΔexoS_pUCP_exoS | Deficient only in ExoS; complemented with a wild-type version of exoS | This study |
| PAO1 ΔexoS_pUCP_exoS(E381D) | Deficient only in ExoS; complemented with exoS with mutant ADP-r activity domain | This study |
| PAO1 ΔexoS_pUCP_exoS(R146K) | Deficient only in ExoS; complemented with exoS with mutant Rho-GAP activity domain | This study |
| PAO1 ΔexoS_pUCP_exoS(E379D/E381D/R146K) | Deficient only in ExoS; complemented with exoS with mutant ADP-r and Rho-GAP domains | This study |
| PAO1 ΔexoS_pUCP_exoS(Δ51-77) | Deficient only in ExoS; complemented with exoS with mutant membrane localization domain | This study |