FIG. 3.

Gb3 is present in the submucosal vasculature, and Gb4 is abundant on the cell surface and throughout colonic epithelial cells. Freshly thawed human colonic sections were incubated with monoclonal anti-Gb3 (A) or polyclonal anti-Gb4 (B) and stained with the appropriate secondary Alexa Fluor 488 antibody. Sections were counterstained with 0.01% Evans blue dye to accentuate tissue structure. (C to F) HCT-8 cells were grown to equivalent densities on coverslips (C and D) and were stained to detect Gb3 (E) or Gb4 (F) as described above.