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Gb3 and Gb4 synthase transcripts are present in normal colonic epithelia. (A and B) A freshly thawed human colonic section was subjected to a modified H&E staining procedure to visualize tissue structure (A), followed by isolation of epithelial cells by LCM (B). (C and D) Gb3 synthase and Gb4 synthase mRNA levels in LCM-isolated epithelial cells (epithelium) and the remaining colonic section (epithelium + r) (C) or HCT-8 cells and Vero cells (D) were assessed by qRT-PCR.
