FIG. 7.

Gb4 synthase RNAi knockdown decreases cell surface Gb4 expression and increases cell surface Gb3 expression. (A) A stable HCT-8 Gb4 synthase-reduced (knockdown) cell line was generated, and the Gb4 synthase transcript level was measured by qRT-PCR. (B to E) Gb3 and Gb4 cell surface levels in HCT-8 wild-type and Gb4 synthase-reduced cells were evaluated by fluorescence microscopy. Error bars represent the range of values for three experiments. Abbreviations: scr, scrambled; kd, knockdown; WT, wild type.