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Stx2 binding to HCT-8 Gb4 synthase-reduced cells is decreased compared to that to HCT-8 wild-type cells. HCT-8 wild-type and Gb4 synthase-reduced cells were intoxicated with 200 ng/ml Stx1 (A and B) or Stx2 (C and D), and toxin was detected with cognate mouse monoclonal antibody and an Alexa Fluor 488-conjugated anti-mouse IgG antibody.
