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ANCCA is required for cancer cell proliferation and E2F target gene expression. (A, B) Asynchronously growing SKBR3 and HeLa cells were transfected with siRNAs targeting ANCCA or a control sequence and harvested different days after transfection for cell numeration. Data are representative of results from triplicate experiments. (C, D) Cells transfected as above were harvested for Western blotting (WB) or RT-PCR 48 h after transfection.
