FIG. 6.

Overexpressed ANCCA is required for chromatin assembly of E2Fs and the HCF-1-MLL hKMT complex and correlates with cancer cell proliferation. (A to C) SKBR3 cells were transfected with siRNA targeting ANCCA, E2F1, or the control sequence and harvested at 24 h after transfection for Western blotting (A) or ChIP (B and C) with the indicated antibodies. (D, E) Sections of breast tissues from 58 patients were subjected to IHC with ANCCA or Ki67 antibodies. The correlation between the IHC scores of ANCCA and Ki67 was analyzed by a Student t test with the coefficient of correlation and P values indicated.