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DNA binding assays of wild-type (WT) and mutant Cdc13N. (A) EMSA of wild-type Cdc13N with the 11-mer DNA that binds the DBD of Cdc13 with a 3 pM affinity. (B) EMSA of WT Cdc13N with a 43-mer single-stranded yeast telomeric DNA. (C) Structure of Cdc13N showing single alanine mutants tested for DNA binding as red stick models. (D) EMSA of WT Cdc13N and K73A, K75A, V133A, T140A, and F142A single mutants with the 43-mer DNA.
