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. 2010 Sep 22;84(23):12300–12314. doi: 10.1128/JVI.01607-10

FIG. 2.

FIG. 2.

The postentry restriction in SIRC cells prevents HIV-1 cDNA synthesis and can be overcome by an HIV-1 (H/SCA) chimera. (A, B) Titration of VSV-G HIV-1 and HIV-1 (H/SCA) GFP vectors. The percentages of infected SIRC (rabbit) or HeLa (human) cells 3 days postinfection are plotted as a function of the titer of the viral inoculum. The cumulative data for the HIV-1 vector in panel A are identical to those shown in Fig. 1A. (C) The consecutive quantification of virus entry (flow cytometry-based virion fusion assay), reverse transcription (quantitative PCR for late RT products), nuclear import (quantitative PCR for 2-LTR circles), and gene expression (flow cytometry for GFP-positive cells) of SIRC cells infected with VSV-G HIV-1 or HIV-1 (H/SCA) GFP vectors carrying BlaM-Vpr was carried out in principle as reported previously (67). Shown are the arithmetic means ± SEM of results from four independent experiments.