HSV-1 blocks ATR signaling independently of ICP0. (A) HeLa cells were infected with HSV-1 or d106 at an MOI of 10, and 3 mM HU was added at the indicated times postinfection and incubated for another 2 h. Western blotting was performed for P-Chk1 S345, total Chk1, and ICP0. (B) HeLa cells were infected with KOS, θβ, or d106 at an MOI of 5, 3 mM HU was added at 6 h postinfection, and samples were collected 2 h later. Western blotting was performed as for panel A with the addition of ICP4.