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mATRIP R&D does not detect ATRIP. (A) HeLa cells were mock transfected or transfected with HA-ATRIP. Western blotting was performed with HA, rATRIP Upstate, and mATRIP R&D. Actin served as a loading control. (B) Vero cells were infected twice with lentiviruses expressing shRNA to GFP or ATR. Knockdown was determined by Western blotting for ATR N19, rATRIP Upstate, and Chk1. (C and D) Knockdown was also determined by immunofluorescence with rATRIP Upstate (C) and mATRIP R&D (D).
