FIG. 6.

ATRIP and RPA but not phospho-RPA are in stage II microfoci. Vero cells were infected with HSV-1 at an MOI of 2 for 6 h. Infected cells were treated with the helicase-primase inhibitor BAY 57-1293 to generate stage II microfoci. (A and B) Cells were fixed and stained with rATRIP Upstate and monoclonal ICP8 (A) or monoclonal RPA and rabbit ICP8 367 (B). (C) Vero cells were either mock infected and treated with 2 mM hydroxyurea for 6 h or infected with HSV-1 at an MOI of 10 for 6 h in the presence of BAY 57-1293. Cells were fixed and stained for RPA and phosphorylated RPA.