FIG. 5.

Effects of gB:N/T on EGFR-specific infection by transiently retargeted HSV. (A) Schematic representation of gD:wt and retargeted gD:3C/Δ711/38C-scEGFR. SP, signal peptide; TM, transmembrane region; CT, cytoplasmic domain; α-EGFR scFV, anti-EGFR single-chain antibody; aa, amino acids. (B) Vero cells were transfected with expression plasmids for the gD proteins indicated to the right and then infected with K-gB:wtΔgD or K-gB:N/TΔgD (indicated to the left) at MOIs of 5, followed by treatment with acidic buffer. Equal volumes of supernatant collected the next day were used for infection of the cells indicated at the top. Cells were immunostained for ICP4 at 6 hpi.