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. 2010 Sep 22;84(23):12362–12374. doi: 10.1128/JVI.00712-10

FIG. 1.

FIG. 1.

Z inhibits C/EBPα- and C/EBPβ-mediated activation of the TNFR1 promoter (TNFR1p). (A) (Left panel) HeLa cells were transfected with a TNFR1p-CAT construct in the presence or absence of cotransfected Z, C/EBPα, or control vectors. The relative CAT activity for each condition is shown; the value for the activity of the promoter construct plus the vector control is set at 1. Values are given as means ± standard deviations of results from three independent experiments. (Right panel) Immunoblot analyses were performed to determine the amounts of C/EBPα and Z in the extracts used for the CAT assays. (B) (Left panel) HeLa cells were transfected with TNFR1p-CAT in the presence or absence of cotransfected Z or C/EBPβ expression vectors as indicated. (Right panel) Immunoblot analyses were performed to determine the amounts of C/EBPβ and Z in the extracts used for the CAT assays.