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. 2010 Sep 22;84(23):12362–12374. doi: 10.1128/JVI.00712-10

FIG. 5.

FIG. 5.

The effect of Z on C/EBPα- and C/EBPβ-mediated activation is promoter dependent. (A) (Left panel) HeLa cells were transfected with the Ob-luciferase construct in the presence or absence of Z or C/EBPα expression vectors as indicated. The relative luciferase activity for each condition is shown; the value for the activity of the Ob-luciferase construct plus the vector control DNA is set at 1. Values are given as means ± standard deviations of results from two independent experiments. (Right panel) Immunoblot analyses were performed to determine the amounts of C/EBPα and Z in the extracts. (B) HeLa cells were transfected with a Zp-luciferase construct in the presence or absence of various combinations of the wt Z, mutant Z(A204D), and C/EBPα expression vectors. The relative luciferase activity for each condition is shown; the value for the activity of the Zp-luciferase construct plus the vector control DNA is set at 1. Values are given as means ± standard deviations of results from two independent experiments performed in duplicate.