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Z does not bind directly to the TNFR1 promoter. An EMSA was performed by incubating in vitro-translated Z, C/EBPα, C/EBPβ, or reticulocyte lysate control with 32P-labeled DNA probes containing either a consensus C/EBP binding sequence (left panel) or the TNFR1 promoter sequence from position −94 to position −75 (right panel). Protein-DNA complexes are indicated by arrows.
