FIG. 2.

Far-Western blot analysis showing the interaction of N with ribosomal proteins from the 40S subunit. To reduce the nonspecific background, the experiment was carried out at increasing salt concentrations. Representative Western blots at three NaCl concentrations, 140 mM (A), 200 mM (B), and 500 mM (C), are shown. The single band from the PVDF membrane, shown in panel C, was excised and analyzed by mass spectrometry. The LC/MS/MS study detected peptides matching three ribosomal proteins, RPS13, RPS17, and RPS19 (D). The exact locations of the peptides in RPS19 are shown at the bottom.