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. 2010 Sep 27;30(23):5514–5530. doi: 10.1128/MCB.00390-10

FIG. 1.

FIG. 1.

Nonfermentable carbon sources stimulate pseudohyphal growth in WT a/α diploid SK1 strains (AMP 1618 × AMP 1619 transformed with pRS316). Identical results were obtained with another diploid WT strain (AMP 109) (cf. Fig. 1 and 2). Colony morphology (A), agar invasion (B), and ascus formation (C) are shown after growth for 7 days. (D) Stimulation of pseudohyphal growth in respiration-deficient petite cells. The colony morphology after 7 days of growth is shown. (E) Stimulation of pseudohyphal growth by ethanol. (F) Asci formed by pseudohyphal cells contain four viable spores. Asci formed by a WT strain (AMP109) on SLA medium supplemented with 2% KOAc were dissected with a tetrad dissection microscope, and spores were placed onto a YPD plate and allowed to germinate and grow for 2 days. (G) PCR genotyping of the mating type locus reveals a 2:2 segregation pattern for MATa and MATα.