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. 2010 Sep 27;30(23):5514–5530. doi: 10.1128/MCB.00390-10

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FIG. 2. — Ime1 and the protein kinase activity of Ime2 are required for pseudohyphal growth. The colony morphology of WT (AMP 109) and ime1Δ/ime1Δ (AMP 115) strains (A) and of WT (MSY 135-43 × MSY 136-40) and ime2Δ/ime2Δ (MSY 202-14 × MSY 203-27) strains (B) is shown. Bar graphs show percentages of pseudohyphal colonies. For each strain and carbon source, >200 colonies were classified as pseudohyphal or nonpseudohyphal. Error bars represent standard errors. (C and D) Agar invasion by WT (AMP 109) and ime1Δ/ime1Δ (AMP 115) strains (C) and by WT (MSY 135-12 × MSY 138-17) and ime2Δ/ime2Δ (MSY 203-22 × MSY 206-36) strains (D). Filamentation (E) and agar invasion (F) of WT (KSY 187) and K97R-ime2/K97R-ime2 (KSY 162) strains were scored after 7 days of growth.