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. 2010 Sep 27;30(23):5514–5530. doi: 10.1128/MCB.00390-10

FIG. 7.

FIG. 7.

cAMP signaling is hyperactive in Σ1278b cells compared to SK1 cells. (A) Sporulation of a/α diploid SK1 (AMP 109; filled bars) and Σ1278b (MLY 61 a/α; open bars) cells. (B) Induction of heat shock genes in WT cells (AMP 109 and MLY 61 a/α) shifted from 30°C to 39°C for 30 min. (C and D) Accumulation of glycogen (C) and trehalose (D) in WT cells (AMP 109 [filled bars] and MLY 61 a/α [open bars]) shifted from 25°C to 37°C for the indicated times. (E) Expression of constitutively active Ras2G19V from plasmid pMW2 in a WT a/α diploid SK1 strain (AMP 109) inhibits sporulation. Filled bars, AMP 109 plus pRS316; open bars, AMP 109 plus pMW2. (F) Deletion of GPR1 in a WT a/α diploid Σ1278b strain (MLY 61 a/α) increases sporulation. Filled bars, WT (MLY 61 a/α); open bars, gpr1Δ/gpr1Δ strain (MLY 232 a/α). (G and H) Glycogen accumulation is shown for cells shifted from 25°C to 37°C for the indicated times. (G) AMP 109 transformed with pRS316 (filled bars) or pMW2 expressing Ras2G19V (open bars). (H) MLY 61 a/α (black bars), MLY 232 a/α (gpr1Δ/gpr1Δ; gray bars), and MLY 187 a/α (ras2Δ/ras2Δ; open bars). (I and J) Trehalose accumulation in the cells shown in graphs G and H. For each measurement, the average and standard error for two replicates are shown.