FIG. 5.
Flash-induced absorption changes for the six purified H. marismortui rhodopsins. The purified proteins were dissolved in MES buffer containing 0.05% n-dodecyl-β-d-maltoside, pH 5.8, and transient absorbance changes were recorded at their corresponding ground state λmax. (A) Transients were measured for HmBRI at 550 nm (top), HmBRII at 550 nm (middle), and HmHR at 580 nm (bottom), and all photocycles were completed within a millisecond. (B) Transients were measured for HmSRI at 570 nm (top), HmSRII at 480 nm (middle), and HmSRM at 500 nm (bottom), and photocycles were completed with a second. The curves represent the change of absorbance corresponding to the λmax for each rhodopsin upon green-laser (532-nm) excitation.