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Western blot of natural and modified PilA-PhoA fusions in the presence of PilO. The antibodies used were a monoclonal antibody specific for E. coli PhoA (A); monoclonal antibody 5.44, which was specific for a P. aeruginosa 1244 pilin epitope (B); and monoclonal antibody O11, which was specific for P. aeruginosa IATS serotype O11 (C). All plasmids were expressed in P. aeruginosa PA103wzyPaO11::aacC1 containing pUCP26PilO. See the legend to Fig. 2 for construct details.
