FIG. 2.

(A) The fpaA and msvR transcripts generated using the T203 template in the absence (−) or presence (+; 200 nM) of MsvR. Mutations were made in two putative TATA boxes (T255 and T256) for fpaA, and transcripts generated using those templates are displayed (with and without MsvR). (B) The msvR and fpaA transcripts generated from T203 in the presence of increasing concentrations (0 nM, 20 nM, 40 nM, 100 nM, and 200 nM) of MsvR in both standard in vitro transcription assays (solid lines) as well as in an order-of-addition assay (dotted lines), in which the general transcription factors (TBP/TFB) were incubated with the template prior to the addition of MsvR. The amount of transcript produced from promoters in each reaction was quantitated and compared to the respective control reaction (taken as 100% transcript). The data displayed are averages of two independent experiments.