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. 2010 Sep 10;192(22):5991–6000. doi: 10.1128/JB.00440-10

FIG. 3.

FIG. 3.

Expression of cspD in spoT and spdR mutant strains. (A) β-Galactosidase activity assay of cspD expression in strains NA1000 (squares) and SP0247 (diamonds) carrying vector pEL4 in PYE medium after different times of growth. (B) Complementation of the spdR mutation on cspD expression. β-Galactosidase activity assay was carried out with C. crescentus strains NA1000 (white bars), SP0247 (dark gray bars), SP0247(pCA39) (carrying the wild-type [wt] spdR gene) (light gray bars), and SP0247(pCA40) [carrying the mutated spdR(D64A) gene] (black bars) harboring pEL4. Assays were performed at stationary phase (48 h). (C) β-Galactosidase activity assay of cspD expression in strains NA1000 (white bars), SP0200 (light gray bars), and SP0210 (dark gray bars) carrying vector pEL4 in PYE medium at exponential phase (exp) and stationary phase (24 h and 48 h). (D) Immunoblot analysis using a polyclonal anti-CspD antiserum of total protein extracts obtained from 48-h cultures of the NA1000, spoT, spdR, and spdS strains grown for 48 h. NA, NA1000 extract; spoT, SP0200 extract; spdR, ΔspdR mutant strain (47) extract; spdS, ΔspdS mutant strain (47) extract.