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Phosphate transfer between SpdS and SpdR. The purified kinase domain of SpdS was previously autophosphorylated with [32P]ATP and then was mixed with purified His-SpdR. The reaction was stopped at several time points, and phosphotransfer to SpdR was evaluated by SDS-PAGE. Lane 1, autophosphorylated SpdS (time zero); lanes 2 to 5, products of the phosphotransfer reaction after 0.5, 2, 10, and 30 min, respectively.
