TABLE 2.
Expression of cspD in mutant strains of C. crescentus
| Straina | Disrupted ORFb | Putative ORF productc | β-Galactosidase activity (Miller units)d |
|---|---|---|---|
| NA1000(pEL4) | Wild type | 3,698.7 ± 33.5 | |
| NA1000(pEL5) | Wild type | 740.1 ± 21.0 | |
| 23/11C | CCNA_00247 | Response regulator | 623.0 ± 28.5 |
| 22/7H | CCNA_00254 | RarD family membrane permease | 1,980.3 ± 52.8 |
| 5/3 | CCNA_01611 | Transporter, drug/metabolite exporter family | 2,121.7 ± 125.9 |
| 1/6G | CCNA_02106 | TonB-dependent outer membrane receptor | 1,977.8 ± 120.0 |
| 5/4E | CCNA_02186 | Acetolactate synthase 3 regulatory subunit | 1,740.0 ± 37.7 |
| 24/5E | CCNA_02188 | Cytosolic protein | 1,998.6 ± 47.9 |
| 4/2C | CCNA_02603 | Phosphatidylserine decarboxylase | 1,903.8 ± 5.6 |
| 2/6F | CCNA_02670 | ABC transporter ATP binding protein | 1,022.3 ± 22.5 |
| 46/2A | CCNA_03269 | Glucokinase | 1,863.6 ± 149.2 |
| 30/3C | CCNA_03839 | Acylamino acid-releasing enzyme | 2,102.6 ± 4.8 |
a
The promoter constructs used are shown in parentheses. All mutant strains contained pEL4.
b
Numbers indicate the open reading frame (ORF) designation for C. crescentus NA1000 as deposited in GenBank.
c
Obtained from the sequence databases.
d
Values are averages and standard deviations of data obtained in three experiments. Data refer to measurements at 24-h time point.