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. Author manuscript; available in PMC: 2011 Feb 1.
Published in final edited form as: Gastroenterology. 2009 Oct 1;138(2):649–658. doi: 10.1053/j.gastro.2009.09.055

Figure 4.

Figure 4

The interaction of LPA5 with NHERF2. (A) GST-LPA5 or GST recombinant proteins were overlaid onto membranes containing recombinant NHERF1, NHERF2, and PDZK1. The bottom panel: Ponceau-S—stained membrane. (B) GST-LPA5 bound to the second PDZ domain, PDZ2, of NHERF2. The bottom panel: Ponceau-S. (C) Lysates from PS120/NHERF1 and PS120/NHERF2 cells transfected LPA5 or pcDNA were immunoprecipitated with either an anti-NHERF1 or anti-NHERF2 antibody. The presence of co-immunoprecipitated LPA5 was detected with an anti-HA antibody. The left 2 lanes show the expression of HA-LPA5 in transfected cells. Representative data from 3 independent experiments are shown.