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NHERF2 is necessary for LPA5-mediated stimulation NHE3. NHE3 activity in the absence of presence of LPA was determined. (A) PS120/NHE3V. (B) PS120/NHE3V/LPA5. (C) PS120/NHE3V/LPA5/NHERF2. (D) PS120/NHE3V/LPA5/ NHERF1. (E) PS120/NHE3V/NHERF2. n > 6. *P < .001 compared with the control.