Figure 8.

ATP suppresses cyclic nucleotide-evoked membrane responses in OE slices. A, Representative EOG responses from OE slices attributable to Ringer's solution, odor, and a cyclic nucleotide mixture (100 μm IBMX, 50 μm CPT-cAMP, and 50 μm 8-Br-cGMP). Filled triangles correspond to the time of loop injection of the test solutions. B, Representative on-cell current-clamp recording from an ORN in an OE slice. Various test solutions were superfused onto the slice for 30 sec, indicated by the shaded region. The cell was allowed to recover for 7 min after each test application. Note that the coapplication of ATP (10 μm) and the mixture suppressed the evoked membrane potential changes. C, The electrical activity from each ORN was integrated from baseline, normalized to the initial cyclic nucleotide mixture response, and averaged (means + SEM). ^*p < 0.05, Newman-Keuls post hoc test. n = 3 ORNs from three slices, also indicated in each column.