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Microtubule mixtures of various structural types: (a) Typical morphology of frozen hydrated MTs obtained by polymerization of purified tubulin. A high defocus (∼ -7μm) was used in this picture to enhance the contrast for visualization. Diameter differences are obvious, demonstrating that the polymerized MTs are a mixture of multiple structural types. (b) Distribution of structural types for MTs polymerized with added Taxol (grey bars), or with 20% DMSO and added Taxotere (black bars).
