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. Author manuscript; available in PMC: 2010 Nov 9.

Published in final edited form as: Chembiochem. 2008 Jul 2;9(10):1641–1648. doi: 10.1002/cbic.200800032

Melting curves for modified oligonucleotides before A) and after B) coupling with complementary DNA. [A] = (A_T−A_T0)/A_T0. (A_T0 is the absorbance at 10°C). UV absorption was monitored at 260 nm.

Melting curves for modified oligonucleotides before A) and after B) coupling with complementary DNA. [A] = (A_T−A_T0)/A_T0. (A_T0 is the absorbance at 10°C). UV absorption was monitored at 260 nm.